Signalling

Part:BBa_K1766006

Designed by: Sarah Wideman   Group: iGEM15_Stockholm   (2015-09-10)

MicF regulated LuxI

LuxI is a quorum synthase which produces 3-oxo-hexanoyl homoserine lactones (OHHL). In this construct LuxI has been fused to the ompF 5’UTR. This is the target for micF regulatory RNA. In wild type E. coli micF will inhibit translation of LuxI at high osmolarity conditions. We therefore expect MicF-Regulated-LuxI to produce more OHHL at low osmolarity conditions than at high osmolarity.

Due to time constraints iGEM Stockholm 2015 were unable to construct this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 839
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None