Composite

Part:BBa_K1765002

Designed by: Kinuko Ueno   Group: iGEM15_Tokyo-NoKoGen   (2015-09-07)


PcpcG2-Colicin E1-T0 terminator

This part is coding Colicin E1, which can make pore in the lipid membrane of the E.Coli. In this part, colicin E1 expression is controlled under cpcG2 promoter, green light sensing promoter. Because of this promoter, we can express colicin E1 only when the host bacteria exposed to the light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 549


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Categories
Parameters
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