Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K1765001

Designed by: Kinuko Ueno   Group: iGEM15_Tokyo-NoKoGen   (2015-09-07)


PcpcG2-Colicin E1-T0 terminator

This part is coding Colicin E1, which can make pore in the lipid membrane of the E.Coli. In this part, colicin E1 expression is controlled under cpcG2 promoter, green light sensing promoter. Because of this promoter, we can express colicin E1 only when the host bacteria exposed to the light.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3
    Illegal SpeI site found at 1934
    Illegal PstI site found at 1940
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal SpeI site found at 1934
    Illegal PstI site found at 1940
    Illegal NotI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3
    Illegal SpeI site found at 1934
    Illegal PstI site found at 1940
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3
    Illegal XbaI site found at 18
    Illegal SpeI site found at 1934
    Illegal PstI site found at 1940
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 598


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