Device

Part:BBa_K176243

Designed by: Chao Li, Hao Jiang   Group: iGEM09_USTC   (2009-10-15)


For characterization of K176240: VR7+pCon 0.36->K176240->PoPS

VR7+pCon 0.36 as input for characterization of K176240.

VR7+pCon 0.36->GFP->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
    Illegal NheI site found at 1595
    Illegal NheI site found at 1618
    Illegal NheI site found at 2444
    Illegal NheI site found at 2467
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 726
    Illegal BglII site found at 2286
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3422


[edit]
Categories
Parameters
n/aFor characterization of K176240: VR7+pCon 0.36->K176240->PoPS