Device
Part:BBa_K176242
Designed by: Danqian Liu, Hao Jiang Group: iGEM09_USTC (2009-10-14)
High input score automatic selector (with reporter): PoPS->GFP->K176239
PoPS->GFP->luxI-LVA->tetR-LVA + pCon 0.36->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)
Cell Death = (Cell Density * (pCon 0.36 + Population Average Input PoPS) + Input AHL) / (1 + Input PoPS)
Cells with relative high input score in the population will be selected, cells with relative low input score will die.
The first luxI-LVA is used to calculate average score in the population.
GFP is input score reporter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2270
Illegal NheI site found at 2293
Illegal NheI site found at 3119
Illegal NheI site found at 3142 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1401
Illegal BglII site found at 2961 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4586
Illegal BsaI.rc site found at 662
Illegal BsaI.rc site found at 4097
[edit]
Categories
Parameters
n/a | High input score automatic selector (with reporter): PoPS->GFP->K176239 |