Device

Part:BBa_K176238

Designed by: Danqian Liu, Hao Jiang   Group: iGEM09_USTC   (2009-10-14)


High input score automatic selector: PoPS->luxI-LVA->K176227

PoPS->luxI-LVA->tetR-LVA + pCon 0.01->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)

Cell Death = (Cell Density * (pCon 0.01 + Population Average Input PoPS) + Input AHL) / (1 + Input PoPS)

Cells with relative high input score in the population will be selected, cells with relative low input score will die.

The first luxI-LVA is used to calculate average score in the population.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1524
    Illegal NheI site found at 1547
    Illegal NheI site found at 2373
    Illegal NheI site found at 2396
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 655
    Illegal BglII site found at 2215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3840
    Illegal BsaI.rc site found at 3351


[edit]
Categories
Parameters
n/aHigh input score automatic selector: PoPS->luxI-LVA->K176227