Device
Part:BBa_K176237
Designed by: Chao Li, Hao Jiang Group: iGEM09_USTC (2009-10-14)
Repressible cell density->PoPS: PoPS->tetR-LVA->K176085->PoPS
PoPS->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS
Output PoPS = ((Cell Density * pCon 0.15) + Input AHL) / (1 + Input PoPS)
High Input PoPS -> Low Output PoPS
High Input AHL -> High Output PoPS
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 855
Illegal NheI site found at 878
Illegal NheI site found at 1704
Illegal NheI site found at 1727 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1546
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2682
[edit]
Categories
Parameters
n/a | Repressible cell density->PoPS: PoPS->tetR-LVA->K176085->PoPS |