Device

Part:BBa_K176237

Designed by: Chao Li, Hao Jiang   Group: iGEM09_USTC   (2009-10-14)


Repressible cell density->PoPS: PoPS->tetR-LVA->K176085->PoPS

PoPS->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS

Output PoPS = ((Cell Density * pCon 0.15) + Input AHL) / (1 + Input PoPS)

High Input PoPS -> Low Output PoPS

High Input AHL -> High Output PoPS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 855
    Illegal NheI site found at 878
    Illegal NheI site found at 1704
    Illegal NheI site found at 1727
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1546
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2682


[edit]
Categories
Parameters
n/aRepressible cell density->PoPS: PoPS->tetR-LVA->K176085->PoPS