Device

Part:BBa_K176230

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-10-13)


High input score automatic selector (with reporter): PoPS->GFP->K176229

PoPS->GFP->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)

Cell Death = (Cell Density * (pCon 0.15 + Population Average Input PoPS) + Input AHL) / (1 + Input PoPS)

Cells with relative high input score in the population will be selected, cells with relative low input score will die.

The first luxI-LVA is used to calculate average score in the population.

GFP is input score reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2270
    Illegal NheI site found at 2293
    Illegal NheI site found at 3119
    Illegal NheI site found at 3142
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1401
    Illegal BglII site found at 2961
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4586
    Illegal BsaI.rc site found at 662
    Illegal BsaI.rc site found at 4097


[edit]
Categories
Parameters
n/aHigh input score automatic selector (with reporter): PoPS->GFP->K176229