Coding

Part:BBa_K1760001

Designed by: Anthony Zhao, Seray Cicek, Umar Owadally, Katariina Jaenes, Matt D'lorio, ChungMin Lee, Drupad Rajyaguru   Group: iGEM15_Toronto   (2015-09-18)

TodE


Designed biobrick is a coding sequence that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. TodE expresses Catechol 2-3 dioxygenase protein. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD.


Toronto 2015 Pathway.png


In pseudomonas putida species, HOD is aerobically metabolized through TCA cycle. Theoretically, a E.Coli MG1655 type bacteria containing todC1C2BADEF genes can degrade toluene through TCA cycle by using its own native enzymes.


TodEonly.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]



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Categories
Parameters
None