Part:BBa_K1755003

strong RBS + K1755002

This part was a constitutive expression element for ribB. https://parts.igem.org/Part:BBa_K1755001#references

This part consisting of B0032 and B0014 which were characterized as

ribB coding sequence and double terminators.

In our project, we havea applied kinds of promoters

which are sensitive to the concentration of copper heavy metal ions such as Cu2+ Hg2+ and Cr6+ to regulate the expression of protein ribB.

The existence of ribB will enhance the production of riboflavin.

Moreover, riboflavin, as the electron carrier, could improve the electricity producing performance of the Microbial Fuel Cells (MFCs).

So in this way, we successfully correlated the concentration of heavy metal ions together with the output voltage of the MFC.

When ribB coding sequence had RBS and terminator,we could regulate the expression of protein ribB with different promoters.