Coding

Part:BBa_K1753000

Designed by: Da Di   Group: iGEM15_WHU-Pharm   (2015-09-15)


the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)

this part is the omega subunit of E_coli RNA polymerase, upstream of which is T7 promoter and RBS for in vitro transcription and translation system (NEB PURExpress),downstream is BBa_B0014(double terminator),between the RBS and the terminator is NdeI and NheI restriction site. the total scheme is : T7promoter-RBS-NdeI site-omega subunit-NheI site-BBa_B0014

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None