Regulatory

Part:BBa_K175025

Designed by: Daniel Solis Escalante   Group: iGEM09_TUDelft   (2009-07-28)


PLacI+RBS+TetR+TT+PLambda+RBS+GFP+TT

Plasmid 2 (SDP) in NFF delay circuit. Addition of IPTG target the production of TetR which initiates the negative cascade. the cascade ends with the production of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1775


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