Regulatory

Part:BBa_K175024

Designed by: Sriram Tiruvadi Krishnan   Group: iGEM09_TUDelft   (2009-07-28)


PLacI+Key3c+TT+PLambdain+RBS+GFP+TT

Plasmid 2 (SDP) for the riboregulator delay circuit. The addition of IPTG will induce the production of key3c which allows the translation of cI in the plasmid 1 (BBa_K175023). cI induces the expression of GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 206
    Illegal BsaI.rc site found at 1202


[edit]
Categories
Parameters
None