Composite

Part:BBa_K1748009

Designed by: Thomas Bridge   Group: iGEM15_Westminster   (2015-09-17)


OmcA-His

Part BBa_K1748002 (OmcA) with added His-10 tag. Made using RPD standard and converted into BioBrick format, contains medium strength promoter, medium strength Rbs, coding sequence, cleavable protein linker, His-10 tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 29
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1377
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 946
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 12
    Illegal BsaI.rc site found at 2352


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Categories
Parameters
None