Measurement

Part:BBa_K1747016

Designed by: Harry Bennett   Group: iGEM15_LaVerne-Leos   (2015-08-27)

pLR + hpd + gfp

This part produces both the 4-hydroxyphenylpyruvate dioxygenase (HPD) enzyme as well as green flourescent protein. This part was designed to measure gene expression level in the vector it is put in. HPD catalyzes the reaction that converts 4-hydroxyphenylpyruvate into homogentisic acid, the rate limiting step of tocopherol biosynthesis. Using this part in a vector that has a tocopherol synthesis pathway allows one to characterize the effect of tocopherol upregulation in your vector. It is regulated by part K817002, which acts constitutively without any additional operons. However, when the protein FadR is present, it is positively regulated by fatty acids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 993
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 588
    Illegal BsaI.rc site found at 2025

LaVerneLeos_K1747016.png


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Parameters
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