Composite

Part:BBa_K1747012

Designed by: Harry Bennett   Group: iGEM15_LaVerne-Leos   (2015-08-26)


Fatty acid promoter - GFP - crt Z,E,B,I,Y - term - Repressor circuit

This a complete circuit coding for five genes including GFP, crtE, crtB, crtI, and crtY. This also has a repressor circuit included in order for the promoter to act as a fatty acid concentration regulated manner.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3347
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2883
    Illegal NgoMIV site found at 3013
    Illegal AgeI site found at 2098
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


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Categories
Parameters
None