Intermediate

Part:BBa_K1747006

Designed by: Harry Bennett   Group: iGEM15_LaVerne-Leos   (2015-08-24)

Fatty acid promoter - GFP - Double terminator

This circuit was designed to evaluate the response of the fatty acid promoter using GFP and a double terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734

Usage

This part was created in order to characterize the function of BBa_k817002 in E.coli. Below in the results section figure 1 shows mean of FL1 FACS recording in comparison to a negative control of wild type E.coli.


Results:

LaVerneLeos_K1747006.png

Figure 1


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Categories
Parameters
None