Coding
Part:BBa_K1746002
Designed by: Tushar Nichakawade Group: iGEM15_Cooper_Union (2015-09-11)
TdT GIP 213-215 subAAA (Mutated TdT Variant)
This DNA sequence is a mutation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. To create this sequence, amino acids 215-218 GIP, were substituted with AAA. This sequence was demonstrated to show 63% catalytic activity relative to full length, un-mutated, TdT. Expression of this sequence results in a protein that can be used for 3'-end non-templated synthesis of oligonucleotides.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 154
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1384
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 933
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Categories
Parameters
None |