Coding

Part:BBa_K1746002

Designed by: Tushar Nichakawade   Group: iGEM15_Cooper_Union   (2015-09-11)


TdT GIP 213-215 subAAA (Mutated TdT Variant)

This DNA sequence is a mutation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. To create this sequence, amino acids 215-218 GIP, were substituted with AAA. This sequence was demonstrated to show 63% catalytic activity relative to full length, un-mutated, TdT. Expression of this sequence results in a protein that can be used for 3'-end non-templated synthesis of oligonucleotides.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1384
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 933


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Categories
Parameters
None