Coding

Part:BBa_K1746001

Designed by: Christopher Lastihenos   Group: iGEM15_Cooper_Union   (2015-09-11)

TdT delta26-143 (Truncated TdT Variant)

This DNA sequence is a mutated variant of a sequence coding for the bovine terminal deoxynucleotidyltransferase (TdT) enzyme. The sequence was originally desinged by Repasky et. al in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. It includes a deletion of the 26th to 143rd amino acids of the naturally occurring TdT protein. It shows catalytic activity similar to that of full length TdT in 293T cells. It is intended to be used for adding nucleotides to the 3 prime end of a DNA sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 581


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Categories
Parameters
None