Composite

Part:BBa_K174014

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-14)

cwlJ + sleB, B. subtilis germination genes

Both CwlJ and SleB are germination specific cortex hydrolases [1, 2]. Although SleB has a major effect for the germination, cwlJ's effect is minor. However Bacillus subtilis with double mutation without cwlJ and sleB were not able germinate again [2].

To control the germination rate tightly, cwlJ and sleB genes are combined to make this device.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Moriyama, R., A. Hattori, S. Miyata, S. Kudoh, and S. Makino. 1996. A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis and response of the enzyme to l-alanine-mediated germination. J. Bacteriol. 178:6059-6063
  2. Ishikawa, S., K. Yamane, and J. Sekiguchi. 1998. Regulation and characterization of a newly deduced cell wall hydrolase gene (cwlJ) which affects germination of Bacillus subtilis spores. J. Bacteriol. 180:1375-138
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