DNA

Part:BBa_K174006

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-10)

Sac single crossover site for Bacillus subtilis

When inserted into a Bacillus subtilis integration vector, it provides a single crossover copying the whole plasmid into the bacterial chromosome. Hence this integration biobrick can be ligated with the biobrick that is wanted to be transferred into Bacillus subtilis and inserted into an integration vector such as pmutin4.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None