Deleted
Sample Discontinued
Experience: None
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Part:BBa_K1734011

Designed by: Ana Karen Tello Gómez   Group: iGEM15_Tec-Monterrey   (2015-09-18)

C5 (pPH + HBM signal sequence + Nanoluc + T2A sequence + EGFR + 6xHis)


Usage and Biology

This construct contains the polyhedrin promoter, the Honeybee Melittin signal peptide, a Nanoluc coding sequence with the Histidine Tag, united with the T2A sequence for polycistronic expression with the Epidermal Growth Factor Receptor with a Histidine Tag.

Signal peptide sequences are for extracellular secretion of Nanoluc, RFP remains inside the cell, this to compare extracellular and intracellular protein expression and characterize the functionality of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1096
    Illegal BamHI site found at 699
    Illegal XhoI site found at 864
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1771
    Illegal SapI site found at 623


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Parameters
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