Device

Part:BBa_K1734007

Designed by: Ana Karen Tello Gómez   Group: iGEM15_Tec-Monterrey   (2015-09-18)

C1 (p10 + HBM signal sequence + Nanoluc + T2A sequence + mRFP1 + 6xHis)


Usage and Biology

This construct consists of a promoter p10, the Honeybee Melittin signal peptide, and the Nanoluc coding sequence with a Histidine Tag, a T2A sequence for polycistronic expression, and and finally RFP protein with Histidine Tag.

Signal peptide sequences are for extracellular secretion of Nanoluc, RFP remains inside the cell, this to compare extracellular and intracellular protein expression and characterize the functionality of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 717
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5
    Illegal SapI site found at 641


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Categories
Parameters
None