Coding

Part:BBa_K1734005

Designed by: Eduardo Ramírez Montiel   Group: iGEM15_Tec-Monterrey   (2015-09-18)


C6 (hr5 enhancer + IE1 promoter + HBM signal sequence + Nanoluc + T2A + mRFP1 + T2A + Zeo Resistance

The protein is used mainly as a reporter gene for protein localization and expression in cells. This sequence is optimized for expression in the Sf9 cell line.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 27
    Illegal EcoRI site found at 348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 27
    Illegal EcoRI site found at 348
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 27
    Illegal EcoRI site found at 348
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 27
    Illegal EcoRI site found at 348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 27
    Illegal EcoRI site found at 348
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None