Regulatory

Part:BBa_K1734003

Designed by: Eduardo Ramírez Montiel   Group: iGEM15_Tec-Monterrey   (2015-09-18)

Polyhedrin promoter + Lysozyme’s signal peptide

The biobrick contains the polyhedrin promoter from the baculovirus Autographa californica. After the promoter there is a secretion signal sequence of the protein lysozyme from the organism Gallus gallus (chicken) [1].

Tec_Monterrey_Conf_PPH_CL.jpg

This part has been confirmed by restriction analysis of the pSB1C3 vector

Reference:

[1]Tsuchiya, Y., Morioka, K., Shirai, J., & Yoshida, K. (2004). Structural requirements of signal peptide in insect cell. Nucleic Acids Symposium Series, 48(1), 181-182.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None