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Coding

Part:BBa_K1733099

Designed by: Shaheer Piracha   Group: iGEM15_BostonU   (2015-09-16)


Crisper/spCas9- with NLS

Addition of this NLS sequence tags the protein for transport into the nucleus, so that the Crisper/spCas9 can function optimally in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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