Part:BBa_K1733000
orf7 Recombination Directionality Factor
This part contains orf7, which is the corresponding recombination directionality factor to the TP901-1 integrase. It catalyzes the unidirectional inverse reaction of the TP901-1 integrase, allowing for the inversion, deletion, and cassette exchange of sequences of DNA.
Orf7 recognizes AttL and AttR recombination sites that flank some sequence of interest, and, when both TP901-1 and orf7 are present, the sequence within the recombination sites is can be inverted, deleted, or undergo cassette exchange with another sequence of DNA. After performing the reaction, orf7 will no longer recognize the recombination sites (AttP and AttB), so the sequence will not continually inverting.
To test this part within our project, we used a reporter that contained a gene that codes for a fluorescent protein flanked by the AttL and AttR recombination sites. Originally, this fluorescent protein is unexpressed, but when we add TP901-1 and orf7 to the system, the proteins catalyze the inversion reaction, the sequence in between the recombination sites is flipped, and the fluorescent protein is expressed.
Orf7 and TP901-1 can be used in further applications to create a switch to flip and knock out a gene of interest.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 186
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