Coding

Part:BBa_K1732029

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-18)


FireflyCO-His

Firefly luciferase (BBa_I712019) with 6X-His tagged for easier protein purification. Sequence was codon optimized by IDT codon optimization tools.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 404
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 309
    Illegal AgeI site found at 1579
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/light
Parameters
None