Coding

Part:BBa_K1732024

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-18)


YFPCO-His

YFP (BBa_K1491004) with 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimizing tool.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


[edit]
Categories
//cds/reporter/yfp
//function/reporter/fluorescence
Parameters
colorYellow