Coding

Part:BBa_K1732022

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-18)


EGFPCO-His

EGFP (BBa_1491002) with 6X-His tag for easier protein purification. Original sequence is codon optimized by IDT Codon Optimized Tool for E.coli.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/gfp
//function/reporter/fluorescence
Parameters
colorGreen