Coding

Part:BBa_K1732021

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-17)


BFPCO-His

mTagBFP (BBa_K1491001) with a 6X-His tag for easier protein purification. The sequence was codon optimized by IDT Sequencing for E.coli.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 546
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 457
    Illegal BsaI.rc site found at 622


[edit]
Categories
//cds/reporter
//function/reporter/fluorescence
Parameters
colorBlue