DNA

Part:BBa_K1732016

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-09)

pSB1C3-J23115-B0034-T7RNAP-sites-B0034-YFP

A control used for the estrogen biosensor which has restriction sites in place of the ER-LBD (BBa_K1732023). The sites added the amino acids ACLKLGGSTGGGSHNC between K179 and K180.

Estrogen control sites 2.jpg


Michelle Chart 1.jpg

Although the T7 RNAP is expressed using a weaker constitutive promoter (J23115), it results in the expression of higher levels of fluorescence compared to that the constitutively expressed J23100 promoter, indicating its ability to produce high levels of protein. Thus, if the goal was to synthesis large concentrations of luciferase protein, it would be more efficient to use the T7 system.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3304
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None