Deleted
Sample Discontinued
Experience: None
Not Used
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Part:BBa_K1732001

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-03)


J23100-CDcel-GlucCO-B0015

J23100 Promoter with a RBS (BBa_S04423), CDcel domain, Gaussia princeps luciferase, and a B0015 terminator. Allows Gaussia luciferase to be constitutively transcribed and translated. Luminescence can be expressed by reacting the luciferase product with coelenterazine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1614
    Illegal BamHI site found at 1065
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 805


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Parameters
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