Composite
Trg-EnvZ

Part:BBa_K1731000

Designed by: Alejandro Rodriguez-Gama, Lizbeth Airais Bolaños Castro   Group: iGEM15_UNAM-CU   (2015-09-14)

Constitutive Trg-EnvZ Chimera

The Chimera Trg-EnvZ is part of a two component system and it constitutes a chimeric protein composed of the extracellular and transmembrane domains of chemoreceptor Trg and the cytosolic kinase domain of osmosensor EnvZ . Trg is activated by periplasmic binding proteins, and transduces the message to EnvZ to phosphorylate OmpR, a transcription factor which binds to the OmpC promoter driving transcription of whichever gene that is downstream.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205


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Categories
Parameters
None