Composite

Part:BBa_K173024

Designed by: Lorenzo Pasotti, Susanna Schiavi, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-16)

adhB inducible system (HSL)

This part is a 3OC6-HSL -> alcohol dehydrogenase II inducible system.

Alcohol dehydrogenase II enzyme (adhB gene, BBa_K173017) of Z. mobilis catalyzes the transformation of acetaldehyde into ethanol. This enzyme, as reported in literature, is also able to work in the opposite direction, oxidizing ethanol to acetaldehyde.


The expression of adhB can be induced with 3OC6-HSL autoinducer molecule: it binds luxR protein (encoded by BBa_C0062), which is constitutively expressed by tetR promoter (BBa_R0040). LuxR-HSL complex can work as a transcriptional activator for lux promoter (BBa_R0062).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


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