Composite

Part:BBa_K173022

Designed by: Lorenzo Pasotti, Giulio Chiesa, Manuel Lupotto, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-16)

adhB and pdc inducible system (HSL)

This part is a 3OC6-HSL -> pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adhB) inducible system.

pdc and adhB can engineer an ethanologenic pathway in a suitable host such as E. coli. The expression of pdc and adhB can be induced with 3OC6-HSL autoinducer molecule: it binds luxR protein (encoded by BBa_C0062), which is constitutively expressed by tetR promoter (BBa_R0040). LuxR-HSL complex can work as a transcriptional activator for lux promoter (BBa_R0062).

This inducible system is analogous to BBa_K173021, but with the two ethanologenic gene position cross-exchanged to study this position effect on ethanol production.

This BioBrick can be used to characterize the ethanol-producing activity of BBa_K173020.

Pv B10 2 BBa K173022.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2754
    Illegal AgeI site found at 3960
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


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Categories
Parameters
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