Generator

Part:BBa_K173020

Designed by: Lorenzo Pasotti, Valerio Vitelli, Manuel Lupotto, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-16)

adhB and pdc protein generator

This device takes PoPS as input and produces alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) enzymes.

Pyruvate decarboxylase (pdc, BBa_K173016) catalyses the decarboxylation of pyruvic acid to acetaldehyde and carbon dioxide, Alcohol dehydrogenase II (adhB, BBa_K173017) catalyses the acetaldehyde reduction to ethanol. The latter enzyme, as reported in literature, is also able to work in the opposite direction, oxidizing ethanol to acetaldehyde. The two enzymes come from Zymomonas mobilis ethanologenic bacterium and constitute an essential step of alcoholic fermentation.

So, this device contains the minimum set of genes that are required to engineer a heterologous fermentation pathway.

The coding sequences of pdc and adhB genes have been optimized for Escherichia coli codon usage.

This device is analogous to BBa_K173003, but with the two gene position cross-exchanged to study this position effect on ethanol production.

Pv B6 BBa K173020.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1685
    Illegal AgeI site found at 2891
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None