Part:BBa_K1729001

Yeast mitochondrial ribosomal protein MRPS12

This translational unit includes the Kozak and codon-optimized sequence for the Saccharomyces cerevisiae [http://www.yeastgenome.org/locus/S000005319/overview MRPS12] gene, which encodes a ribosomal protein of the small subunit of the mitochondrial ribosome. The coding sequence begins with a mitochondrial localization signal (MLS), which is presumably removed in the mitochondrion. The resultant protein is a homologue of the highly conserved uS12 ribosomal protein (Ban et al.), which is essential for ribosome function.

The part sequence corresponds to nucleotides 694840-694361 on chromosome XIV of the S. cerevisiae S288C reference genome. See also: BBa_K1729002

Screen capture from SnapGene®

References

Ban, Nenad et al. “A New System for Naming Ribosomal Proteins.” Cur Opin Struct Biol 24 (2014): 165–169.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 442
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]