Signalling

Part:BBa_K1728022

Designed by: Wan-Yun Chiu   Group: iGEM15_CGU_Taiwan   (2015-09-17)

LUCY tag


A recent study in 2011 revealed that the N-terminal 17 amino acid of leucine rich repeat 32 (LRRC32) is a signal peptide for proper surface expression in HEK293 cells and T regulatory cells. The 17 amino acid signal peptide is named Lucy due to leucine repeats.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None