Part:BBa_K1728009

IL1β toehold switch RNA sensor with T7 promoter

IL1β toehold switch RNA sensor with T7 promoter

Usage and Biology

Toehold switch is a secondary structure of RNA. Because of special sequence design, it can form a hairpin structure and hide ribosome binding site (RBS)in the loop. There are three basic elements of toehold switches including 30bp trigger sequence(TTTCCCTTCATCTTTGAAGAAGAACCTATC), bacteria ribosome binding site and linker.The trigger sequence of this part is complementary to a part of Interleukin 1β (IL-1β) mRNA(IL1β partial sequence, BBa_K1728005). Once the IL-1β mRNA is presented, the toehold structure will open. Therefore, the ribosome is able to bind on the RBS and translate the down stream gene.In order to highly transcribe, we add a T7 promoter sequence in front of the toehold switch sequence(IL1β toehold switch RNA sensor, BBa_K1728001).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]