Regulatory

Part:BBa_K1728001

Designed by: Yung-Li Chen, Timothy Chan En Haw   Group: iGEM15_CGU_Taiwan   (2015-09-16)

IL1β toehold switch RNA sensor

IL1β toehold switch RNA sensor


Usage and Biology

Toehold switch is a secondary structure of RNA. Because of special sequence design, it can form a hairpin structure and hide ribosome binding site (RBS)in the loop. There are three basic elements of toehold switches including 30bp trigger sequence(TTTCCCTTCATCTTTGAAGAAGAACCTATC), bacteria ribosome binding site and linker.The trigger sequence of this part is complementary to a part of Interleukin 1β (IL-1β) mRNA(IL1β partial sequence, BBa_K1728005). Once the IL-1β mRNA is presented, the toehold structure will open. Therefore, the ribosome is able to bind on the RBS and translate the down stream gene.


Toehold switch secondary structure prediction

CGU_IL1B.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None