Composite

Part:BBa_K1727008

Designed by: Ying Kuan   Group: iGEM15_TCU_Taiwan   (2015-09-05)

T5+Lac Operator+RBS+Signal peptide+Signiferin+Terminater

Signiferin is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. Signiferin has demonstrated effectiveness in killing Methicillin-Resistant Staphylococcus aureus (MRSA), and has been proven by the TU-Delft 2013 iGEM team. In order to have more efficient to get Signiferin, we treated signal peptide from S.lividans on the upstream of N-terminal of antimicrobial peptides to facilitate the peptide production.

This part is built by part:BBa_K1727000. We used T5 promoter, Lac operator, RBS, terminator on pQE 60 vector. So we can use IPTG induction to get our product.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 79
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 79
    Illegal BglII site found at 259
    Illegal BamHI site found at 253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/antimicrobial
Parameters
None