Part:BBa_K1727005
Signiferin (Crinia signifera)
Mechanism of Signiferin
Signiferin is a kind of antimicrobial peptide (AMPs) and it is a stable peptide that has extensive abilities to kill or inhibit the growth of bacteria. It plays a role in defense mechanism for Crinia signifera to against microbes. Signiferin use its chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Signiferin has demonstrated effectiveness in killing Methicillin-Resistant Staphylococcus aureus (MRSA), and has been proven by the TU-Delft 2013 iGEM team.
Features of Signiferin
1. Small and simple: The sequence of Signiferin is only around 100 bp. For E.coli, 100 base pairs is in the working capacity. And its folding structure is a-helix it will not limited to E.coli translation system.
2. Activity: anti-Gram positive bacteria.
3. Net charge: Its net charge is +2, so it can interact with bacterial cell membrane effectively.
4. Hydrophobic region: Signiferin hydrophobic region rate is 60 %, so it can mix with Vaseline into our medical dressing.
This part is a coding gene from the skin mucus of Crinia signifera.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |