Composite

Part:BBa_K1725431

Designed by: Andrey Filipov   Group: iGEM15_Glasgow   (2015-09-08)


J23110 + RBS + UirR + B0015 + PlsiR + GFP

The UV intensity response regulator coding sequence was positioned downstream of J23110, a constitutive promoter family member, and upstream of the double terminator B0015. In this part the promoter associated with UirR was also added, alongside with GFP, so that activity may be reported.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1389
    Illegal BsaI.rc site found at 2137
    Illegal SapI.rc site found at 510


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Categories
Parameters
None