UirR (UV intensity response Regulator)
UirR is a protein part native to the cyanobacterium, Synechocystis. Along with UirS, the proteins form a chromatic two-component system (Sensor and Regulator) to UV and green light (lambda = 534nm) which activates and represses UirS (K1725410), respectively. The protein is attached to the transmembrane protein UirS, which like its other cyanobacteriochrome analogue, ccaS (K608102) is a histidine kinase. After exposure to unidirectional UV-A, UirS releases UirR, which physically interacts with the lsiR promoter (K1725400), activating transcription. All three parts are required for a cell to respond to the UV-A stimulus.
For UirR with a ribosome binding site check K1725421.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 144
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal SapI.rc site found at 448
Ji-Young Song, Hye Sun Cho et al (2011) "Near-UV cyanobacteriochrome signaling system elicits negative phototaxis in the cyanobacterium Synechocystis sp. PCC 6803" PNAS. DOI: 10.1073/pnas.1104242108
Prabha Ramakrishnan† and Jeffrey J. Tabor (2016) "Repurposing Synechocystis PCC6803 UirS–UirR as a UV-Violet/Green Photoreversible Transcriptional Regulatory Tool in E. coli" ACS Synthetic Biology. DOI: 10.1021/acssynbio.6b00068
Yuu Hirose et al (2008)"Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein." Proc Natl Acad Sci U S A.. 105: 9528–9533.
Tabor, J. J. et al.(2010), " Multichromatic Control of Gene Expression in Escherichia coli", J. Mol. Biol. doi:10.1016/j.jmb.2010.10.038