Reporter
PlsiR.GFP

Part:BBa_K1725401

Designed by: Andrey Filipov   Group: iGEM15_Glasgow   (2015-09-08)


PlsiR + B0034 + GFP

The promoter activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light is upstream of GFP with the B0034 RBS (BBa_I13500). This allows activity in response to UV-A to be measured. For the promoter to be activated UirR and UirS must be present and the cell has to be exposed to UV-A.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 456
    Illegal BsaI.rc site found at 1204


[edit]
Categories
//function/reporter
//function/reporter/fluorescence
Parameters
None