Part:BBa_K1723005

PAM rich URS J23117Alt promoter

PAM rich URS J23117Alt is a new fully synthetic promoter obtained by mutating a model promoter [2], PAM rich URS J23117 promoter (BBa_K1723001) [1]. It acts the exactly same way as the PAM rich URS J23117 promoter but is targeted by others specific sgRNAS. See the design section for more information about its creation. It has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000), that can only bind in presence of a PAM sequence, as a gene transcription regulator, when in complex with one sgRNA targeting the promoter such as: inhibiting sgRNA X0 (BBa_K1723006), activating sgRNA X4 (BBa_K1723007), or inhibiting sgRNA X35 (BBa_K1723008).

This part was experimentally validated, see https://parts.igem.org/Part:BBa_K1723005:Experience

Discover more parts that can work with this one:

http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 277
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 167
  Illegal XhoI site found at 195
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437.

[2] Alec AK Nielsen & Christopher A Voigt (2014). Multi-input CRISPR/Cas circuits that interface host regulatory network. Molecular systems biology, 10(11), 763.