Part:BBa_K1723001

PAM rich URS j23117 promoter

PAM rich URS J23117 is an improvement of the J23117 (BBa_J23117) weak promoter [1]. This promoter is the promoter J23117 flanked with a PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence (URS) to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: the activator sgRNA Z4 (BBa_K1723003), the inhibitor sgRNA Z0 (BBa_K1723002), or the other inhibitor sgRNA Z35 (BBa_K1723004). dCas9 can only bind if the DNA target sequence is preceded by a PAM sequence.

This part was experimentally validated, see https://parts.igem.org/Part:BBa_K1723001:Experience

Discover more parts that can work with this one:

http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 56
  Illegal NheI site found at 79
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 43
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437.