Device

Part:BBa_K1719010

Designed by: Lin Shi   Group: iGEM15_Amoy   (2015-09-05)

Circuit encoding leucine dehydrogenase and formate dehydrogenase

It is a complete device which could express two enzymes leucine dehydrogenase and formate dehydrogenase. When the reaction system was added NADH and trimethylpyruvic acid, leucine dehydrogenase converts the substrate into L-tert-leucine. At the same time, NADH is oxidized to NAD+. While the formate dehydrogenase can convert formate into carbon dioxide. NADH is regenerated. The conversion of TMP is 98.89% and the e.e value of product L-tert-leucine is >99%.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 839
    Illegal NgoMIV site found at 2307
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None