Reporter

Part:BBa_K1718003

Designed by: Hannah Young   Group: iGEM15_CU_Boulder   (2015-09-14)


N-acyl-homoserine-lactone inducible LuxPr promoter generating RFP

The part consists of LuxR followed by the AHL inducible LuxPr promoter which is placed in front of RFP. This part contains BBa_F2620 which is composed of a constitutive TetR promoter which produces the LuxR transcription factor. Followed by that is the LuxPr promoter, which is induced when a N-acyl-homoserine-lactone molecule binds to the LuxR transcription factor. Following this promoter is the reporter RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1642
    Illegal AgeI site found at 1754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


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Categories
Parameters
None